3D PRINTED HYDROGEL GLUCOSE SENSOR ON ARGON PLASMA ACTIVATED POLYSTYRENE

Authors

  • Jens Jüttner Technische Hochschule Aschaffenburg, Würzburger Str. 45, 63743 Aschaffenburg, Germany
  • Nenad Krstic University of Nis, Visegradska 33, 18106 Nis, Serbia
  • Achim Müller
  • Monika Knuth
  • Christiane Thielemann

DOI:

https://doi.org/10.14311/CTJ.2020.2.01

Keywords:

bioprinted sensor, adhesion, Ar plasma, activated polystyrene, glucose measurement

Abstract

This study presents a proof of principle concept for a two-dimensional bioprinted glucose sensor on Petri dishes that allows for glucose measurements in cell culture medium. To improve bioink adhesion, the polystyrene surfaces of standard Petri dishes are activated with argon plasma, which increases roughness and hydrophilicity. The bioink containing the sensor chemistry—namely fluorescently labeled ConA/Dextran embedded in alginate microbeads—was printed on the activated Petri dishes with an extrusion-based bioprinter. The printed sensor showed good stability and adhesive properties on polystyrene. The glucose concentration was examined using a standard fluorescence microscope with filters adapted to the emission wavelength of the donor and reference dyes. The printed glucose sensor showed high sensitivity and good linearity in a physiologically relevant range of glucose concentrations.

Author Biographies

Jens Jüttner, Technische Hochschule Aschaffenburg, Würzburger Str. 45, 63743 Aschaffenburg, Germany

BioMEMS Lab, Faculty of Engineering

Nenad Krstic, University of Nis, Visegradska 33, 18106 Nis, Serbia

Department of Chemistry, Faculty of Science and Mathematics

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Published

2020-06-30

Issue

Vol. 50 No. 2 (2020)

Section

Original Research

License

Copyright (c) 2021 Jens Jüttner, Nenad Krstic

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