RAMAN LABEL-FREE VISUALISATION OF TITANIUM DIOXIDE NANOPARTICLES UPTAKE IN BJ CELL LINES
Keywords:
titanium dioxide nanoparticles, cellular uptake, label-free, confocal Raman microscopyAbstract
Titanium dioxide nanoparticles represent one of the most frequently applied nanomaterials. Due to its advantageous physicochemical properties affecting the final products, use of this nanomaterial in daily used products is increasing. Beside the addition into glaze or enamels, titanium dioxide nanoparticles are found in UV protective cosmetic products applied on skin. According to the studies confirming the potential carcinogenic effect of titanium dioxide nanoparticles application of such nanomaterial may cause health risk. Cellular uptake of nanoparticles and their distribution in cell environment may play an important role in nanoparticles toxicological effect. Thus, evaluation of cellular uptake of nanoparticles is the additional step for evaluation of nanoparticles toxicology. The main objective of this study was to confirm the assumption of the cellular uptake of tested TiO2 nanoparticles using human fibroblasts BJ cell lines and confocal Raman microscopy as a new, promising, label-free imaging technique for studying the distribution of exogenous substances in cells. The results of this study confirm that tested TiO2 nanoparticles are uptaken by cells and distributed in intracellular environment, where form aggregates, possibly during their transport via endocytosis.
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Copyright (c) 2017 Monika Harvanová, Vlastimil Mašek, Dagmar Jírová, Hana Kolářová
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