IMUNOFLUORESCENČNÍ ANALÝZA PROAPOPTICKÝCH SIGNÁLNÍCH MOLEKUL V BUŇKÁCH LIDSKÉHO MELANOMU PO FOTODYNAMICKÉ TERAPII

Robert Bajgar, Hana Kolářová, Svatopluk Binder, Adéla Dašková, Hana Lenobelová, Klára Pížová, Kateřina Tománková

Abstract


IMMUNOFLUORESCENCE ANALYSIS OF PROAPOPTOTIC SIGNALING MOLECULES IN HUMAN MELANOMA CELLS AFTER PHOTODYNAMIC TREATMENT. Photodynamic therapy (PDT) is connected with oxidative damage of biomolecules causing significant impairment of essential cellular functions that lead to cell death. It is the reason, why photodynamic therapy has also found its application in treatment of different oncological, cardiovascular, skin and eye diseases. The cell death after PDT is mediated by an apoptotic and/or necrotic process including activation of various biomolecules. In the presented study we have used immunofluorescence method to detect caspase 3 and 9, poly ADP-ribose polymerase (PARP) in their active forms, and release of the cytochrome c as the proapoptotic protein after photodynamic treatment of human melanoma cells.


Keywords


apoptosis; photodynamic therapy; enzyme-linked immunosorbent assay (ELISA)

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ISSN 0301-5491 (Print)
ISSN 2336-5552 (Online)
Published by the Czech Society for Biomedical Engineering and Medical Informatics and the Faculty of Biomedical Engineering, Czech Technical University in Prague.