IMUNOFLUORESCENČNÍ ANALÝZA PROAPOPTICKÝCH SIGNÁLNÍCH MOLEKUL V BUŇKÁCH LIDSKÉHO MELANOMU PO FOTODYNAMICKÉ TERAPII
Keywords:
apoptosis, photodynamic therapy, enzyme-linked immunosorbent assay (ELISA)Abstract
IMMUNOFLUORESCENCE ANALYSIS OF PROAPOPTOTIC SIGNALING MOLECULES IN HUMAN MELANOMA CELLS AFTER PHOTODYNAMIC TREATMENT. Photodynamic therapy (PDT) is connected with oxidative damage of biomolecules causing significant impairment of essential cellular functions that lead to cell death. It is the reason, why photodynamic therapy has also found its application in treatment of different oncological, cardiovascular, skin and eye diseases. The cell death after PDT is mediated by an apoptotic and/or necrotic process including activation of various biomolecules. In the presented study we have used immunofluorescence method to detect caspase 3 and 9, poly ADP-ribose polymerase (PARP) in their active forms, and release of the cytochrome c as the proapoptotic protein after photodynamic treatment of human melanoma cells.
Downloads
Published
Issue
Section
License
Copyright (c) 2017 Robert Bajgar, Hana Kolářová, Svatopluk Binder, Adéla Dašková, Hana Lenobelová, Klára Pížová, Kateřina Tománková
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of the first publication with the work simultaneously licensed under a Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) that allows others to share the work with an acknowledgment of the work's authorship and initial publication in CTJ.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal’s published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website or ResearchGate) prior to and during the submission process, as it can lead to productive exchanges.
CTJ requires that all of the content of the manuscript has been created by its respective authors or that permission to use a copyrighted material has been obtained by the authors before submitting the manuscript to CTJ. CTJ requires that authors have not used any copyrighted material illegally, as for example a picture from another journal or book, a photo, etc. It is the author’s responsibility to use only materials not violating the copyright law. When in doubt, CTJ may ask the authors to supply the pertinent permission or agreement about the use of a copyrighted material.
The opinions expressed in CTJ articles are those of authors and do not necessarily reflect the views of the publishers or the Czech Society for Biomedical Engineering and Medical Informatics.
