FOTOTOXICKÝ VLIV PORFYRINOVÝCH SENSITIZERŮ A VIDITELNÉHO ZÁŘENÍ NA GRAM-POZITIVNÍ METHICILIN-REZISTENTNÍ KMEN S. AUREUS

Adela Hanakova, Katerina Bogdanova, Katerina Tomankova, Klara Pizova, Jakub Malohlava, Svatopluk Binder, Robert Bajgar, Katerina Langova, Milan Kolar, Jiri Mosinger, Hana Kolarova

Abstract


The use of antimicrobial photodynamic therapy (aPDT) as a therapeutic modality for the treatment of localized microbial infections represents an developing new field. The emergence of strains resistant to antibiotics has provided the necessary impulse for new drug or technology discoveries to combat these resistant compounds. Although the aPDT is still in infancy, its need is still growing. Like PDT, main components of antimicrobial photodynamic therapy are appropriate light, dye called photosensitizer and created reactive oxygen species. In this article photosensitizers TMPyP and ZnTPPS4 are investigated for antimicrobial photodynamic therapy. We tested these porphyrins on bacterial methicilin – resistant strain MRSA alone and bound in complex created with hp-β-cyclodextrin. The light emitting diodes (414 nm) were used at the doses 0 and 150 J/cm2. Tested concentrations were from 0.78 to 100 μM. This experimental work predicated that TMPyP is very successful compound in aPDT. In contrary to ZnTPPS4 which was efficient for eradication of tested gram-positive bacteria only in higher concentrations.

Keywords


antimicrobial photodynamic therapy; porphyrins; blue light; light emitting diod

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ISSN 0301-5491 (Print)
ISSN 2336-5552 (Online)
Published by the Czech Society for Biomedical Engineering and Medical Informatics and the Faculty of Biomedical Engineering, Czech Technical University in Prague.