THE EFFECT OF DOCETAXEL ON MOLECULAR MELTING PROFILE OF DNA EXTRACTED FROM HUMAN BREAST ADENOCARCINOMA MCF-7 CELLS

Marianna Trebuňová, Ján Rosocha, Galina Laputková, Mario Jančošek, Jozef Živčák

Abstract


Aim: The aim of this study was to evaluate the genotoxic effect of docetaxel on the human adenocarcinoma MCF-7 cells to detect genetic variations and discover potential associations between the drug and the genotoxic damage of MCF-7 cells. Materials and Methods: High resolution melt analysis (HRM) of genomic DNA isolated from MCF-7 cells was used. Two primers of MDR1 gene were selected: 5´-TGGGGCTTTTAGTGTTGGAC-3´, 5´-TGTGGAGAGCTGGATAAAGTCA-3´. Results: The significant alterations in the melting temperature Tm of DNA treated with docetaxel in the concentrations of 1 μmol/l and 250 μmol/l were observed. Comparing G+C/A+T ratios the increase of the relative content of G, C was detected. Minor changes of the nucleotide content were observed when compared the sequences of DNA for the control and the docetaxel treated group. Conclusions: Using MDR1 primer pairs, our results confirmed that MCF-7 cells are susceptible to genomic DNA instability when exposed to docetaxel.

Keywords


MCF-7 cell line; high-resolution melting analysis; docetaxel

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This work is licensed under a Creative Commons Attribution 4.0 International License.

ISSN 0301-5491 (Print)
ISSN 2336-5552 (Online)
Published by the Czech Society for Biomedical Engineering and Medical Informatics and the Faculty of Biomedical Engineering, Czech Technical University in Prague.