ANALYSIS OF ULTRASOUND FIELD PARAMETERS DURING SONICATION EXPERIMENTS IN VITRO—INFLUENCE OF LABORATORY GLASS AND PLASTICS
Keywords:
ultrasound, ultrasound field, sonication, in vitro experiment, ultrasound dosimetryAbstract
Apart from being a powerful medical imaging technique ultrasound can also be used as a therapeutic modality. In vitro sonication experiments performed on cultured cells are one of primary research methods. However present sonication protocols and methods meet many effects influencing the final ultrasound dose experienced by the sonicated samples. The main aim of this study is to assess the influence of laboratory glass and plastics on ultrasound field parameters during in vitro sonication experiments. We performed measurements of ultrasound field parameters (ultrasound intensity and its local distribution) behind commonly used laboratory glass and plastics placed into the far field region of an ultrasound transducer. We tested the influence of several types of culture dishes, culture plates and sample test tubes. Culture dishes reduced ultrasound intensity by tens of percent but did not affect the shape of ultrasound field. 6-well plate reduced ultrasound intensity only by 5 %. Culture plates with well diameter smaller than the diameter of the main lobe of ultrasound beam focus ultrasound energy. Laboratory glass and plastics with curved surface also focus ultrasound energy. We proved that laboratory glass and plastics considerably affect ultrasound field parameters. Thus sonicated samples are exposed to different ultrasound conditions compared to those reported in some of scientific articles. Rest of factors (standing waves formation, streaming, cell mixing, heating and homogeneity of ultrasound field in terms of near and far ultrasound field) affecting the ultrasound field parameters experienced by sonicated samples also need to be studied further.
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Copyright (c) 2018 Martin Snehota, Jaromir Vachutka, Ladislav Dolezal, Hana Kolarova, Zuzana Mala, Ludmila Zarska
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